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. 2016 Nov 15;30(22):2538–2550. doi: 10.1101/gad.289256.116

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© 2016 Deluz et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 7. — SOX2 is required at the M–G1 transition to promote neuroectodermal differentiation. (A) Immunofluorescence analysis of BRACHYURY (top) and SOX1 (bottom) expression in the SBR cell line after 4 d of differentiation in N2B27. Bars, 20 µm. (B) Flow cytometry of the SBR cell line expressing dox-inducible SNAP-MD-Sox2 (SBR MD) or SNAP-MD*-Sox2 (SBR MD*) after 3 d of differentiation in N2B27 with different doses of dox. (ES wt) Negative control. (C) Quantification of six independent flow cytometry experiments as shown in B, expressed as fold change in the fraction of eGFP⁺ cells and normalized over the values obtained without dox. (*) P < 0.05. (Blue bars) SNAP-MD-Sox2; (red bars) SNAP-MD*-Sox2. (D) Flow cytometry of the SBR cell line expressing dox-inducible SNAP-MD-Sox2 or SNAP-MD*-Sox2 after 4 d of differentiation with different doses of dox in N2B27 with the addition of 3 µM CHIR after 48 h to induce mesendodermal differentiation. (ES wt) Negative control. (E) Quantification of seven independent flow cytometry experiments as shown in D, expressed as fold change in the eGFP⁺/mCherry⁺ ratio and normalized over the value of this ratio without dox. (Blue bars) SNAP-MD-Sox2; (red bars) SNAP-MD*-Sox2. (*) P < 0.05.