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. 2016 Nov 15;30(22):2551–2564. doi: 10.1101/gad.287953.116

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© 2016 Wada et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — The constitutively active Rag complex rescues TFE3 cytoplasmic retention in the absence of FLCN. (A) Schematic of mTORC1 activation by FLCN and subsequent phosphorylation of TFE3 by mTORC1. (B) Immunofluorescence staining of TFE3. Constitutively active or inactive RagB and RagD (HA-tagged) were overexpressed in FLCN-deficient preadipocytes. (Green) RagB/D (HA); (red) TFE3; (blue) DAPI. (C) Quantification result of B. (D) Constitutively active (CA) RagB or RagD was individually overexpressed in FLCN knockout preadipocytes. (Green) RagB or RagD (HA); (red) TFE3; (blue) DAPI. Arrowheads indicate nuclei of RagB/D transfected cells. (*) P < 0.05 versus nontransfected control. Values are represented as mean ± SEM. Bars, 50 µm.