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. 2016 Nov 15;30(22):2551–2564. doi: 10.1101/gad.287953.116

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© 2016 Wada et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — Codeletion of TFE3 with FLCN reverses the browning of white fat. (A) Breeding scheme to obtain FLCN/TFE3 double-deficient male mice. (B) H&E staining of iWAT from animals of the noted genotypes. Bar, 100 µm. (C–F) relative mRNA expression from brown/beige fat-specific genes (C), nuclear encoded mitochondrial genes (D), mitochondria-encoded mitochondrial genes (E), and creatine futile cycle genes (F). (*) P < 0.05; (**) P < 0.01 versus FLCN^lox/lox. n = 8 per group. Values are represented as mean ± SEM.