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. 1977 Nov;24(2):564–579. doi: 10.1128/jvi.24.2.564-579.1977

Physical organization of subgroup B human adenovirus genomes.

C Tibbetts
PMCID: PMC515968  PMID: 916027

Abstract

Cleavage sites of nine bacterial restriction endonucleases were mapped in the DNA of adenovirus type 3 (Ad3) and Ad7, representative serotypes of the "weakly oncogenic" subgroup B human adenoviruses. Of 94 sites mapped, 82 were common to both serotypes, in accord with the high overall sequence homology of DNA among members of the same subgroups. Of the sites in Ad3 and Ad7 DNA, fewer than 20% corresponded to mapped restriction sites in the DNA of Ad2 or Ad5. The latter serotypes represent the "nononcogenic" subgroup C, having only 10 to 20% overall sequence homology with the DNA of subgroup B adenoviruses. Hybridization mapping of viral mRNA from Ad7-infected cells resulted in a complex physical map that was nearly identical to the map of early and late gene clusters in Ad2 DNA. Thus the DNA sequences of human adenoviruses of subgroups B and C have significantly diverged in the course of viral evolution, but the complex organization of the adenovirus genome has been rigidly conserved.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Flint J. The topography and transcription of the adenovirus genome. Cell. 1977 Feb;10(2):153–166. doi: 10.1016/0092-8674(77)90211-2. [DOI] [PubMed] [Google Scholar]
  2. Fujinaga K., Sekikawa K., Yamazaki H. Method for determination of nucleotide sequence homology between viral genomes by DNA reassociation kinetics. J Virol. 1975 Mar;15(3):466–470. doi: 10.1128/jvi.15.3.466-470.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Garon C. F., Berry K. W., Hierholzer J. C., Rose J. A. Mapping of base sequence heterologies between genomes from different adenovirus serotypes. Virology. 1973 Aug;54(2):414–426. doi: 10.1016/0042-6822(73)90153-0. [DOI] [PubMed] [Google Scholar]
  4. Garon C. F., Berry K. W., Rose J. A. A unique form of terminal redundancy in adenovirus DNA molecules. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2391–2395. doi: 10.1073/pnas.69.9.2391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Green M. Oncogenic viruses. Annu Rev Biochem. 1970;39:701–756. doi: 10.1146/annurev.bi.39.070170.003413. [DOI] [PubMed] [Google Scholar]
  6. La S., Green M. The mechanism of viral carcinogenesis by DNA mammalian viruses: DNA-DNA homology relationships among the "weakly" oncogenic human adenoviruses. J Gen Virol. 1967 Oct;1(4):413–418. doi: 10.1099/0022-1317-1-4-413. [DOI] [PubMed] [Google Scholar]
  7. Lewis J. B., Atkins J. F., Anderson C. W., Baum P. R., Gesteland R. F. Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1344–1348. doi: 10.1073/pnas.72.4.1344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McAllister R. M., Nicolson M. O., Reed G., Kern J., Gilden R. V., Huebner R. J. Transformation of rodent cells by adenovirus 19 and other group D adenoviruses. J Natl Cancer Inst. 1969 Oct;43(4):917–923. [PubMed] [Google Scholar]
  9. Meyer J., Neuwald P. D., Lai S. P., Maizel J. V., Jr, Westphal H. Electron microscopy of late adenovirus type 2 mRNA hybridized to double-stranded viral DNA. J Virol. 1977 Mar;21(3):1010–1018. doi: 10.1128/jvi.21.3.1010-1018.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mulder C., Arrand J. R., Delius H., Keller W., Pettersson U., Roberts R. J., Sharp P. A. Cleavage maps of DNA from adenovirus types 2 and 5 by restriction endonucleases EcoRI and HpaI. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):397–400. doi: 10.1101/sqb.1974.039.01.051. [DOI] [PubMed] [Google Scholar]
  11. Mulder C., Sharp P. A., Delius H., Pettersson U. Specific fragmentation of DNA of adenovirus serotypes 3, 5, 7, and 12, and adeno-simian virus 40 hybrid virus Ad2+ND1 by restriction endonuclease R.EcoRI. J Virol. 1974 Jul;14(1):68–77. doi: 10.1128/jvi.14.1.68-77.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Neuwald P. D., Meyer J., Maizel J. V., Jr, Westpahl H. Early gene expression of adenovirus type 2: R-loop mapping of mRNA and time course of viral DNA, mRNA, and protein synthesis. J Virol. 1977 Mar;21(3):1019–1030. doi: 10.1128/jvi.21.3.1019-1030.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ortin J., Scheidtmann K. H., Greenberg R., Westphal M., Doerfler W. Transcription of the genome of adenovirus type 12. III. Maps of stable RNA from productively infected human cells and abortively infected and transformed hamster cells. J Virol. 1976 Nov;20(2):355–372. doi: 10.1128/jvi.20.2.355-372.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pettersson U., Mulder C., Deluis H., Sharp P. A. Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A. 1973 Jan;70(1):200–204. doi: 10.1073/pnas.70.1.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pettersson U., Tibbetts C., Philipson L. Hybridization maps of early and late messenger RNA sequences on the adenovirus type 2 genome. J Mol Biol. 1976 Mar 15;101(4):479–501. doi: 10.1016/0022-2836(76)90241-2. [DOI] [PubMed] [Google Scholar]
  16. Philipson L., Pettersson U., Lindberg U. Molecular biology of adenoviruses. Virol Monogr. 1975;14:1–115. doi: 10.1007/978-3-7091-8391-5_1. [DOI] [PubMed] [Google Scholar]
  17. Roberts R. J. Restriction endonucleases. CRC Crit Rev Biochem. 1976 Nov;4(2):123–164. doi: 10.3109/10409237609105456. [DOI] [PubMed] [Google Scholar]
  18. Robinson A. J., Bellett J. D. A circular DNA-protein complex adenoviruses and its possible role in DNA replication. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):523–531. doi: 10.1101/sqb.1974.039.01.064. [DOI] [PubMed] [Google Scholar]
  19. Roychoudhury R., Jay E., Wu R. Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res. 1976 Jan;3(1):101–116. doi: 10.1093/nar/3.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sambrook J., Williams J., Sharp P. A., Grodzicker T. Physical mapping of temperature-sensitive mutations of adenoviruses. J Mol Biol. 1975 Sep 25;97(3):369–390. doi: 10.1016/s0022-2836(75)80046-5. [DOI] [PubMed] [Google Scholar]
  21. Shaltiel S., Er-El Z. Hydrophobic chromatography: use for purification of glycogen synthetase. Proc Natl Acad Sci U S A. 1973 Mar;70(3):778–781. doi: 10.1073/pnas.70.3.778. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tibbetts C., Johansson K., Philipson L. Hydroxyapatite chromatography and formamide denaturation of adenovirus DNA. J Virol. 1973 Aug;12(2):218–225. doi: 10.1128/jvi.12.2.218-225.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tibbetts C., Pettersson U. Complementary strand-specific sequences from unique fragments of adenovirus type 2 DNA for hybridization-mapping experiments. J Mol Biol. 1974 Oct 5;88(4):767–784. doi: 10.1016/0022-2836(74)90398-2. [DOI] [PubMed] [Google Scholar]
  24. Tibbetts C., Pettersson U., Johansson K., Philpson L. Relationship of mRNA from productively infected cells to the complementary strands of adenovirus type 2 DNA. J Virol. 1974 Feb;13(2):370–377. doi: 10.1128/jvi.13.2.370-377.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Westphal H., Meyer J., Maizel J. V., Jr Mapping of adenovirus messenger RNA by electron microscopy. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2069–2071. doi: 10.1073/pnas.73.6.2069. [DOI] [PMC free article] [PubMed] [Google Scholar]

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