Figure 4.

Accumulation of HERC2 substrates involved in DNA repair. (a) Analysis of apoptotic and late apoptotic/necrotic cells and of caspase-3 activity following UVB-irradiation. (b) Analysis of the repair kinetics of 6-4PPs (6-4 photoproducts) and CPD (cyclobutane pyrimidine dimers) after UVB irradiation. Less sensitivity to apoptosis is observed in patient’s cells with slower relative caspase activity and increased rate of removal of CDP photolesions. No difference was observed upon 6,4PP removal. Results are expressed as the mean±SD of 4 independent experiments.