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. 2016 Dec 16;6:38854. doi: 10.1038/srep38854

Figure 1. Evaluation of TALEN-mediated modification of B2m in pig parthenogenetic embryos.

Figure 1

(a) Schematic diagram of pig B2M protein coding region and the targeting locus of B2m-TALENs. Red arrow and green arrow indicate left arm and right arm of B2m-TALENs, respectively. (b) The PCR products covering B2m-TALEN target site amplified from genomic DNAs of individual injected PA embryos. #1~#6 are the parthenogenetically activated(PA) embryos injected with B2m-TALEN mRNAs, and Cont is the wild-type PA embryo as control. (c) T7EN1 cleavage assay of PCR products covering the targeting site (b). #1~#6 are the parthenogenetically activated(PA) embryos injected with B2m-TALEN mRNAs, and Cont is the wild-type PA embryo as control. (d) Sequencing results of the modified B2m alleles detected in pig parthenogenetic embryos. Embryo #5 and #6 are two examples. Sequences complementary to left arm and right arm of B2m-TALENs are labeled in red and green; mutations, blue, lower case. (e) Chromatographs of sequencing modified B2m alleles in parthenogenetic embryos in which overlapped peaks were observed. Embryo #2 is a wild-type sample as control and #1 and #4 are two examples with overlapped peaks near targeting site. Red arrow and green arrow indicate the targeting sites of B2m-TALEN left arm and right arm, respectively.