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. 2016 Dec 16;6:39444. doi: 10.1038/srep39444

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Copyright © 2016, The Author(s)

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(A) Immunofluorescence observation of localization of the MRN complex to the PCV2 replication center. At 24 and 48 h postinfection, Mre11, p-Nbs1, and Rad50 were determined for colocalization with PCV2 ORF1 with their respective antibodies. Nuclei were visualized by DAPI staining. (B) Western blotting of PCV2-infected cells. The PCV2-infected cells were collected at the indicated times postinfection and were analyzed by immunoblotting for the levels of Mre11, p-Nbs1, and Rad50 by use of their respective antibodies. β-actin was acted as the loading control.