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. 2016 Dec 13;7:13792. doi: 10.1038/ncomms13792

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Copyright © 2016, The Author(s)

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Images of Trh-GAL4-positive dsx neurons in the abdominal ganglion in male flies without (a) and with (b) sbb knockdown. The genotypes of flies were w; Trh-GAL4/UAS>stop>mCD8::GFP; dsx^FLP/+ (a) and w; Trh-GAL4/UAS-sbbRNAi tubP>GAL80>; dsx^FLP/UAS>stop>mCD8::GFP (b), respectively. Scale bar, 50 μm. (c) Trh-GAL4-positive dsx neurons are immunopositive for an anti-5-HT antibody. Scale bar, 50 μm. (d) Trh knockdown in dsx-positive neurons completely blocks copulation. (e) Block of synaptic outputs from serotonergic dsx neurons by TNT inhibits copulation. Inactive TNT (TNTⁱⁿ) has no effect. (f) Copulation defects are rescued by feeding adults on 5-HTP-supplemented foods. 5-HTP was administered at 100 mM according to ref. 20. The mean±s.e.m. and the number of flies examined are shown. ***P<0.001 by the χ²-test.