Figure 1. FISH analysis of wMel-transfected and wild-type B. tabaci with Portiera-specific probe (red) and Wolbachia-specific probe (blue).

(a–d) Wild-type female adult; (e–h) Transfected female adult; (i–l) Wild-type egg; (m–p) Transfected egg. (a,e,i,m) Portiera channel only; (b,f,j,n) Wolbachia channel only; (c,g,k,o) Merged images showing overlap of Wolbachia and Portiera channels in dark field; (d,h,l,p) Merged images showing overlap of Wolbachia and Portiera channels in bright field.