Table 4. Verification of gene expression changes by qRT-PCR.
| Gene | Dorset | HanBB | Han++ | Forward primer | Reverse primer | |||
|---|---|---|---|---|---|---|---|---|
| NR0B1 | 1.0188 ± 0.0226 | 1.996 ± 0.0623 | *** | 0.7014 ± 0.0410 | *** | AACTCAACAGATACTGAGCGAA | GATGAATCTCAGAAGGAAGAGG | |
| KCNJ3 | 1.0512 ± 0.3961 | 0.8816 ± 0.0497 | 1.0615 ± 0.2276 | GTCATTGCTCATTCAAACACC | TTCCATTGTGTTACTCTGCTT | |||
| GDF5 | 1.0193 ± 0.2503 | 0.2573 ± 0.0820 | * | 0.8122 ± 0.1883 | CGTAAGTGGGAGAGAACTTGA | CAATAGGTGAAGAGGAAGTCG | ||
| MED21 | 1.0097 ± 0.1691 | 1.3000 ± 0.2092 | 0.8883 ± 0.2619 | GTCTCTTTGTTTGCGGAGG | CAGCACTCCAATGGCGTTA | |||
| XLOC_017093 | 1.0025 ± 0.0866 | 1.5436 ± 0.1754 | ** | 0.8212 ± 0.1357 | TATCACTGGAGGCGTGTC | AAACCACTGCGTGCCTTA | ||
| LOC101109655.1 | 1.0017 ± 0.0703 | 2.0147 ± 0.0655 | *** | 0.9594±0.0168 | CAGGTACACTTTCAAGGGC | CTCTTTGGAACTATTCCATGC | ||
| LOC101116211 | 1.0113 ± 0.1822 | 0.0525 ± 0.0008 | ** | 0.0693 ± 0.0045 | ** | GCGGGGCACCCTTTGAT | GGTGGAGAAAAGAGGCTGAATAACT | |
| MYH15 | 1.0085 ± 0.1647 | 0.3177 ± 0.0744 | ** | 0.6657 ± 0.1285 | * | TCTGTAGTCTGTATGAGGAACG | CTCACCATTCTCACTCCG | |
| XLOC_001041 | 1.0000 ± 0.0975 | 6.3695 ± 0.1302 | *** | 13.4393 ± 0.0299 | *** | TCCTGGGCACTGACCAAGAG | GAAATGAATGGCAGATCACGAAC | |
| NYAP1 | 1.0095 ± 0.1742 | 0.2894 ± 0.1420 | ** | 0.9249 ± 0.2414 | GTGCTGAATAAGGGCTGT | CAGGAGATGCTGGCAAAG | ||
| BCORL1 | 1.0000 ± 0.1802 | 2.3958 ± 0.0609 | 2.9237 ± 0.1192 | * | GGATGGTGATGTGGTCTTCAACTT | GAGTCTGGTTGCCACTTGTTCTTAC | p < 0.001*** | |
| C-JUN | 1.0000 ± 0.0257 | 3.5769 ± 0.1107 | * | 5.7455 ± 0.0632 | *** | AAGATGGAAACGACCTTCTACGAC | GGGTCATGCTCTGCTTCAGAAT | p < 0.05* |
| ZNF300 | 1.0000 ± 0.1112 | 3.8084 ± 0.0491 | ** | 4.2318 ± 0.1023 | * | GAAACCCTACAAATGTGCTCAGTG | AGGTCTTCCCACAAGCAGTACAT | p < 0.01** |