Table 3. The bioconversion features of pyridine and pyrazine derivatives for which reactions products were detectable.
Substrate |
Product |
|||
---|---|---|---|---|
Name | [M+H]+ | [M+H]+ | NMR | Name/Possible outcome |
Pyridine | 80 | 96 | − | Hydroxylated at the 1-position |
2-Methylpyridine | 94 | 110 | − | Hydroxylated at the 1-position |
3-Methylpyridine | 94 | 110 | − | Hydroxylated at the 1-position |
4-Methylpyridine | 94 | 110 | − | Hydroxylated at the 1-position |
Pyrazine | 81 | 97 and 113 | + | pyrazine-1-oxide and pyrazine-1,4-dioxide |
For the NMR analysis approximately 10 mg of purified bioconversion product was used.