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. 2016 Dec 16;6:39123. doi: 10.1038/srep39123

Figure 3. BRU does not affect the transcriptional level of HIF-1α in hypoxic conditions.

Figure 3

(A) HCT116 cells were cultured under normoxia or hypoxia (1% O2) in the presence of various concentrations of BRU for 12 h. The mRNA levels of HIF-1α were analyzed by qRT-PCR and normalized with β-actin mRNA levels. (B) HCT116 cells were cultured under normoxia or 200 μM CoCl2-induced mimic hypoxia in the presence of various concentrations of BRU for 12 h. The mRNA levels of HIF-1α were analyzed by qRT-PCR and normalized with β-actin mRNA levels.