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. 2016 Dec 15;11:57. doi: 10.1186/s12995-016-0145-2

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Increase in AM mROS production resulting from exposure to UF particles, when compared to coarse particles, was confirmed using MitoSOX™ Red fluorescence. Data was collected on approximately 80 to 100 cells per stage position, with eight to ten stage positions in each of the separate experiments for 180 min. Data were analyzed using NIS Elements (Nikon Inc., Melville, NY). Data from three independent analyses for each particle size were used in the statistical calculations. Stage positions in which the particles did not result in AM generation of ROS were not used in the statistical analysis

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