Table 2.

Intra- and inter-assay reproducibility for the detection of C. fetus

Genome copies/reaction	Intra-assay variations		Inter-assay variations
	Mean Ct (from – to)	CV (from – to)	Mean Ct	CV
1 × 101	-a	-	-	-
1 × 102	36.57–37.69	0.97–2.1	37.13	2.19
1 × 103	33.68–34.11	0.48–1.15	33.89	1.05
1 × 104	30–30.07	0.25–0.16	30.03	0.23
1 × 105	26.37–26.46	0.14–0.27	26.41	0.26
1 × 106	22.62–22.86	0.18–0.7	22.74	0.73
1 × 107	19.02–19.23	0.5–0.83	19.12	0.86

CV coefficient of variation of Ct values [%]

^aCt value out of dynamic range