Table 3.

Summary of average enzymes per bead for prototype assay calibration using Quanterix standard for assay evaluation and clinical test batches

	AEB
Theoretical concentration, pg/ml	0.023	0.069	0.206	0.617	1.85	5.6	16.7	50.0
Run 1	0.006	0.008	0.010	0.023	0.112	0.794	5.951	27.615
Run 2	0.005	0.006	0.008	0.018	0.086	0.692	5.214	23.725
Run 3	0.005	0.007	0.009	0.018	0.089	0.703	5.774	29.431
Run 4	0.006	0.006	0.008	0.020	0.102	0.720	5.665	28.302
Run 5	0.005	0.006	0.008	0.018	0.096	0.720	5.603	28.131
Run 6	0.006	0.007	0.009	0.019	0.089	0.624	5.082	27.607
Run 7	0.011	0.012	0.014	0.025	0.091	0.618	4.674	25.809
Run 8	0.007	0.009	0.011	0.015	0.107	0.580	5.454	24.691
Run 9	0.007	0.009	0.010	0.018	0.084	0.608	5.409	24.587
N (total number of runs)	9	9	9	9	9	9	9	9
Inter-assay mean	0.006	0.008	0.010	0.019	0.095	0.673	5.425	26.655
SD	0.00	0.00	0.00	0.00	0.01	0.07	0.39	2.00
CV%	26.4	24.8	19.1	15.4	10.4	10.3	7.2	7.5

AEB Average enzyme per bead, CV% Coefficient of variation percentage

In runs 6–8, calibration curves prepared using both Quanterix and Fujirebio standards were included