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. Author manuscript; available in PMC: 2016 Dec 16.
Published in final edited form as: Methods Mol Biol. 2012;799:197–216. doi: 10.1007/978-1-61779-346-2_13

Fig. 2.

Fig. 2

Schematics of the optical tweezers assay. (a) Schematic of the optical tweezers. (b) Schematic of the two extreme positions of the bead during calibration. (c) Representation of the motion of the coverglass and the resulting motion of the bead at two different frequencies. Frequencies and amplitudes of the bead motion for each frequency can be measured on the trace of the bead motion. (d) An example of calibration of the trap: the slope of the graph of the viscous drag force vs. bead displacement gives the stiffness of the trap.