Fig. 3.
Downstream signalling of DB-mediated GLP-1 secretion in NCI-H716 cells, (a–c) GLP-1 and PYY secretion in response to DB, PMA and PKC inhibition, (d) GLP-1 secretion in the presence of forskolin (FSK) or DB, with and without PDE inhibition by BMX. (e) DB-mediated GLP-1 secretion in the presence of PKC or ERK1/2 inhibition with U73122orPD98059, respectively, (f) Representative immunoblot of ERK phosphorylation in response to increasing concentrations of DB: bars denote the pERK signal normalised to that of total ERK. Results are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001