Figure 4.

BSCFAs and SCFAs decrease phosphorylation of PKB in primary rat and human adipocytes. Primary rat or human adipocytes were stimulated with insulin (INS) in the absence (CTRL) or presence of 3 and 10 mM of SCFA or BCFA for 10 minutes. Homogenates were subjected to immunoblot analysis and membranes were probed with antibodies against PKB, PKB at serine 473 (S473) and PKB at threonine 308 (T308) as well as β-actin. For primary rat adipocytes, results (n = 3-6) are shown for isobutyric acid (I-BA) and isovaleric (I-VA) (A, B). For human adipocytes, results (n = 3–4) are shown for BA, PA, I- BA and I-VA (C, D). Representative blots are shown.