Table 4.
Isobutyric acid potentiates insulin-stimulated glucose uptake in primary rat adipocytes. Glucose uptake was measured after 30 minutes of stimulation without (BASAL) or with 1 nM insulin (INS) in the absence (CTRL) or presence of 1, 3 and 10 mM isobutyric acid (I-BA), isovaleric acid (I-VA) and acetic acid (AA). The values for I-BA, I-VA or AA are related to respective CTRL (set to 1), either in a basal or an insulin-stimulated state. Mean ± SD (n = 6-7) were used and significance levels were accepted when *p < 0.05, **p < 0.01 and ***p < 0.001. a2.9-fold, b2.8-fold, c2.1-fold increase in insulin-stimulated glucose uptake compared to BASAL.
| 1 I-BA |
3 I-BA |
10 I-BA |
|||||
|---|---|---|---|---|---|---|---|
| CTRLMean | Mean | SD | Mean | SD | Mean | SD | |
| BASAL | 1 | 1.29* | 0.19 | 1.3** | 0.18 | 1.24* | 0.21 |
| INS | 1a | 1.24*** | 0.05 | 1.27*** | 0.1 | 1.25** | 0.11 |
| 1 I-VA |
3 I-VA |
10 I-VA |
|||||
| |
CTRLMean |
Mean |
SD |
Mean |
SD |
Mean |
SD |
| BASAL | 1 | 0.94 | 0.12 | 0.95 | 0.05 | 0.69** | 0.1 |
| INS | 1b | 1.02 | 0.03 | 0.74 | 0.2 | 1.01 | 0.12 |
| 1 AA |
3 AA |
10 AA |
|||||
| |
CTRLMean |
Mean |
SD |
Mean |
SD |
Mean |
SD |
| BASAL | 1 | 0.98 | 0.09 | 0.87 | 0.14 | 0.82** | 0.1 |
| INS | 1c | 0.98 | 0.06 | 1.06 | 0.11 | 1.14 | 0.21 |