Table 5.
BSCFAs and SCFAs decrease phosphorylation of PKB in primary rat and human adipocytes. Rat adipocytes were stimulated without (CTRL) or with INS in the absence or presence of 3 and 10 mM isobutyric acid (I-BA) and isovaleric acid (I-VA) for 10 minutes (n = 3). Human adipocytes were stimulated without (CTRL) or with INS in the absence or presence of 10 mM I-BA or I-VA for 10 minutes (n = 3) or 3 mM and 10 mM propionic acid (PA) or butyric acid (BA) for 10 minutes (n = 2-3). Homogenates were subjected to immunoblot analysis and membranes were probed with antibodies against PKB and PKB at serine 473 (S473) as well as β-actin and quantification was made using Image Lab Software (Bio-Rad Laboratories). Data are presented as fold of isoproterenol (ISO).Mean ± SD were used and significance levels were accepted when *p < 0.05, **p < 0.01 and ***p < 0.001.
| PKBS473 in primary rat adipocytes1 | ||||||
|---|---|---|---|---|---|---|
| + INS |
||||||
| n = 3 | CTRL | INS | 10 I-BA | 10 I-VA | ||
| Mean | 0.09*** | 1 | 0.45** | 0.46** | ||
| SD | 0.07 | 0 | 0.14 | 0.14 | ||
| PKBS473 in human adipocytes2 | ||||||
| + INS |
||||||
| n = 3 |
CTRL |
INS |
10 I-BA |
10 I-VA |
||
| Mean | 0.04*** | 1 | 0.60** | 0.65* | ||
| SD | 0.04 | 0 | 0.10 | 0.22 | ||
| PKBS473 in human adipocytes2 | ||||||
| + INS |
||||||
| n = 2#-3 |
CTRL |
INS |
3 BA# |
10 BA |
3 PA# |
10 PA |
| Mean | 0.02*** | 1 | 0.84 | 0.30*** | 0.98 | 0.35*** |
| SD | 0.12 | 0 | 0.13 | 0.14 | 0.27 | 0.05 |