Figure 2.

Performance of the original protocol, carried out as published.⁵ 3T3-L1 adipocytes were fixed on a 6-well plate (35 mm well diameter) in 10% formaldehyde for 1 hour and stained with 0.2% oil red O in 57% 2-propanol for 2 hours at room temperature. After washing, plates were dried at 32°C as needed, and dye was eluted with 1 ml/well of 100% 2-propanol, which was removed immediately by gentle pipetting. 200 µl of eluate from each well were transferred to a microtiter plate. Scatter plot of photometric absorption vs. relative amount of cells. R² = 0.953, p = 1.179e-10 by linear regression with second-order polynomial regression (optimum fit).