Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Pre-incubation of purified hERα with E2-BSA competes for estradiol binding. Purified ERα was incubated with E2 (solid line) or E2-BSA (dotted line) for four hours before (a) or concurrently with (b) the addition of labeled E2. Incubation was continued for another 2 h at room temperature, and at the end of this period, specific binding was determined by adsorption, removal, and counting of free labeled E2.
