Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Stability of E2-BSA-FITC. E2-BSA-FITC (10-8 M in estrogen) was placed in empty wells or wells containing MC7 cells and incubated for 30 minutes at 4°C. E2-BSA-FITC was detected by reverse phase HPLC using a methanol-water gradient from 80% methanol to 50% methanol over 30 minutes at 1.0 ml/min. The assay was run in triplicate. Representative spectra are shown for E2-BSA-FITC alone (A) and E2-BSA-FITC incubated with MC7 cells (B).
