Table 1.
Template | Primers | Product Length | Melting Temperature | Hot Measurement | Fidelity | n |
18S | gag gtt cga aga cga tca ga (sense) | 316 bp | 83.2 ± 02°C | 78°C | 1.89 ± 0.02 | 17 |
tcg ctc cac caa cta aga ac (antisense) | ||||||
KCNJ10 | tgg tgt ggt gtg gta tct gg (sense) | 411 bp | 83.2 ± 02°C | 78°C | 1.86 ± 0.02 | 15 |
tga agc agt ttg cct gtc ac (antisense) | ||||||
KCNQ1 | ttt gtt cat ccc cat ctc ag (sense) | 239 bp | 82.5 ± 02°C | 79°C | 1.85 ± 0.02 | 17 |
gtt gct ggg tag gaa gag (antisense) | ||||||
KCNQ4 | ccc gga aac cct tct gtg tc (sense) | 245 bp | 83.2 ± 02°C | 80°C | 1.87 ± 0.01 | 17 |
aaa gat gag cac cag gaa cc (antisense) |