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. 2004 Aug 19;32(15):4448–4461. doi: 10.1093/nar/gkh748

Figure 3.

Figure 3

Replication of BPV1 L1 and tRNASer(CGA) genes in CHO L1 and Cos1 L1 cells. (A) PCR analysis of DNA prepared from CHO L1 or Cos1 L1 cells, confirming that the cell lines retained a BPV1 L1 expression construct. (B) Southern blot analysis of Hirt DNA for BPV1 L1 and tRNASer(CGA) gene replication in both CHO L1 and Cos1 L1 cells. 20 μg Hirt DNA samples prepared from CHO L1 or Cos1 L1 cells either untransfected or transfected with a tRNASer(CGA) expression plasmid were restricted with BamHI and EcoRI plus DpnI for at least 4 h and electrophoresed on a 1.0% agarose gel and blotted onto nylon membrane. The Southern blot was hybridized with a mixture of 32P-labeled BPV1 L1 and tRNASer(CGA) gene probes.