Figure 9.
Effects of the tRNASer(CGA) expression construct on expression of L1 proteins encoded by codon-modified PV L1 genes (BPV1 HB L1 and HPV6b HB L1) in CHO and Cos1 cells. (A) L1 mRNA levels. CHO or Cos1 cells were transiently transfected with PV HB L1 gene construct only, or with PV HB L1 gene construct and the tRNASer(CGA) gene. (B) L1 protein expression. Four dilutions of protein samples from cells either transfected with PV HB L1 gene construct only, or co-transfected with PV HBL1 gene construct and the tRNASer(CGA) gene were loaded onto SDS-PAGE gel for protein fractionation and blotted on PVDF membranes for immunoblotting assay. Monoclonal antibody against BPV L1 protein was used to probe the blots. Upper panels show the results of L1 immunoblotting assay; lower panels show the results of β-tubulin immunoblotting assay indicating equal loading of the protein samples between two treatments.