Table 2. T_m values for the triplexes shown in Figure 2B, determined by UV melting (x = BAU).

	pH 6.5				pH 7.0
	x.GC	x.CG	x.AT	x.TA	x.GC	x.CG	x.AT	x.TA
TFO-1	—	—	19.5	—	—	—	—	—
TFO-2	15.6	—	28.2	—	—	—	21.0	—
TFO-3	29.5	15.2	43.6	17.0	—	—	31.3	−
TFO-Con1	—	—	—	—	—	—	—	—

The values are averages of the values determined from the melting and annealing curves. Dashes indicate T_m below 15°C. The experiments were performed in 10 mM sodium phosphate (pH 6.5 or 7.0), containing 1 mM EDTA and 200 mM NaCl. T_m values are accurate to within 0.5°C.