Figure 5.

Pol δ and Pol ε activity on undamaged and damaged substrates containing a 3′ T:G mispair. (A) Gel image of primer extension/excision products for a series of substrates containing undamaged templates. (B) Gel image of primer extension/excision products for a series of substrates containing a TT dimer in the positions indicated by carets. All substrates contain a T:G mispair at the 3′ T of the di-thymine sequence. A diagram of the substrates used is given below (B), with the lines indicating where the primer terminus is for each set of lanes. Each panel of five lanes is further identified by the substrate name described in the legend to Figure 1A. Reactions were performed as described in the legend to Figure 2. Bands marked with an asterisk are primers excised back to the −2 position.