Table 1. Comparison of V-D SJs.
| WT | Core RAG1 | Core RAG2 | |
|---|---|---|---|
| Vβ14-Dβ1 | |||
| Precise joints | 43/48 (90%) | 40/49 (82%) | 29/47 (62%) |
| Imprecise joints | 5/48 (10%) | 9/49 (18%) | 18/47** (38%)a |
| N addition | 5/5 (100%) | 9/9 (100%) | 15/18 (83%) |
| Coding sequences | 0/5 (0%) | 0/9 (0%) | 3/18 (17%) |
| Deletion | 0/5 (0%) | 0/9 (0%) | 3/18 (17%) |
| Microhomology | 0/5 (0%) | 0/9 (0%) | 2/18 (11%) |
| Vβ8.3-Dβ1 | |||
| Precise joints | 47/50 (94%) | 42/49 (86%) | 32/48 (67%) |
| Imprecise joints | 3/50 (6%) | 7/49 (14%) | 16/48** (33%)a |
| N addition | 3/3 (100%) | 7/7 (100%) | 15/16 (94%) |
| Coding sequences | 0/3 (0%) | 0/7 (0%) | 8/16 (50%) |
| Deletion | 0/3 (0%) | 0/7 (0%) | 12/16 (75%) |
| Microhomology | 0/3 (0%) | 0/7 (0%) | 0/16 (0%) |
| Vδ5-Dδ2 | |||
| Precise joints | 55/66 (83%) | 54/67 (81%) | 15/56 (27%) |
| Imprecise joints | 11/66 (17%) | 13/67 (19%) | 41/56** (73%)a |
| N addition | 11/11 (100%) | 13/13 (100%) | 23/41 (56%) |
| Coding sequences | 0/11 (0%) | 1/13 (8%) | 1/41 (2%) |
| Deletion | 0/11 (0%) | 0/13 (0%) | 37/41 (90%) |
| Microhomology | 0/11 (0%) | 0/13 (0%) | 13/41 (32%) |
Number of each joint out of total number analyzed was shown. The frequency of each joint is shown in parentheses. Significant differences versus WT mice, when compared by chi-squared test, are indicated by ** (P < 0.01). Junctions were sequences from two independent animals, and the results were combined to calculate the number of each case.
aDifferences from WT mice are still statistically significant even when the data containing coding sequences are excluded, to exclude possible non-12/23 HJ from SJ (see Discussion).