Skip to main content
. 2004 Aug 27;32(15):4609–4617. doi: 10.1093/nar/gkh790

Figure 3.

Figure 3

Reduction of target mRNA levels in HeLa cells induced by siRNAs targeting MGC29643, GPR39 and GAPDH (A–C). Quantitative real-time RT–PCR was carried out to determine the extent of target mRNA reduction induced by siRNAs. Mean fluorescence is reported from six independent transfection experiments. Error bars show variations among the experiments as the standard deviation of the mean. The extent of target mRNA reduction is measured by the percentage of the transcript level in siRNA-transfected cells relative to the transcript level in mock-transfected cells. (A) Reduction of MGC29643 (solid bars) and GPR39 (empty bars) mRNA levels induced by siRNAs directed against MGC29643. (B) Reduction of MGC29643 (solid bars) and GPR39 (empty bars) mRNA levels induced by siRNAs directed against GPR39. (C) Reduction of GAPDH mRNA level induced by siRNAs directed against GAPDH. (D) RT–PCR and gel electrophoresis of amplified products. The sizes of the amplified products are 402 bp (MGC29643), 220 bp (GPR39) and 280 bp (GAPDH). Lane 1: 100 bp ladder DNA size marker. Lane 2: no-template RT–PCR negative control. Lane 3: HeLa cells transfected with buffer instead of siRNA. Lane 4: HeLa cells transfected with nonspecific siRNA. Lane 5: HeLa cells transfected with MGC-1 siRNA. Lane 6: HeLa cells transfected with MGC-3 siRNA. Lane 7: HeLa cells transfected with MGC-4 siRNA.