Skip to main content
. 2004 Aug 27;32(15):4585–4595. doi: 10.1093/nar/gkh800

Figure 2.

Figure 2

Dimerization of HIV-2 RNAs. (A) RNA fragments corresponding to the first 561 nt of genomic, Nef, Rev or Tat HIV-2 RNAs were incubated for 15 min in monomer (lanes 1–4) or dimer (lanes 5–12) buffer and loaded onto a TBM gel run at 4°C. In lanes 9–12, the RNAs were dimerized in presence of a 20-fold excess of the asPBS antisense oligonucleotide. asPBS binds to the 5′ end of the PBS and nucleotides upstream and inhibits the PBS-dependent dimerization of HIV-2 RNA fragments (15,16). (B) Plot of dimerization as a function of incubation buffer and the presence of asPBS oligonucleotide at 37°C. The y-axis error bars represent the standard deviation of two experiments as shown in (A).