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. 2004 Aug 27;32(15):4585–4595. doi: 10.1093/nar/gkh800

Figure 5.

Figure 5

Dimerization of LTR-spliced HIV-2 RNAs at 37°C. (A) Genomic, Nef, Rev or Tat HIV-2 1–561 RNAs without the LTR intron (nt 61–202) were incubated for 15 min in monomer (lanes 1–4) or dimer (lanes 5–12) buffer and loaded onto a TBM gel run at 4°C. In lanes 9–12, the RNAs were dimerized in the presence of a 20-fold excess of the asPBS antisense oligonucleotide. (B) Plot of dimerization as a function of incubation buffer and the presence of asPBS oligonucleotide at 37°C. Stippled, closed and hatched bars represent the percentage of dimers in monomer buffer or in dimer buffer without or with asPBS, respectively. The y-axis error bars represent the standard deviation of two experiments shown in (A).