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. 2004 Aug 27;32(15):4585–4595. doi: 10.1093/nar/gkh800

Figure 6.

Figure 6

Effect of the absence of the LTR intron on tight dimerization of genomic, Nef, Rev and Tat RNAs. (A) Genomic, Nef, Rev or Tat HIV-2 1–561 RNAs with (odd-numbered lanes) or without (even-numbered lanes) the sequence 61–202 corresponding to the LTR intron, were incubated in dimer buffer at 55°C. After incubation for 30 min, RNAs were subjected to electrophoresis on a TBE agarose gel run at 28°C. (B) Plot of the percentage of tight dimerization at 55°C. Closed bars represent the dimer level of LTR-unspliced RNAs and open bars represent the dimer level of LTR-spliced constructs. The y-axis error bars represent the standard deviation of two experiments shown in (A).