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. 2004 Aug 27;32(15):4585–4595. doi: 10.1093/nar/gkh800

Figure 7.

Figure 7

Effects of antisense oligonucleotides against the ψ-SL1 region on tight dimerization of LTR-spliced RNAs. (A) LTR-spliced 1–561 RNAs were incubated in dimer buffer at 55°C without or with a 20-fold molar excess of asψ, asSL1 or both oligonucleotides, and subjected to TBE electrophoresis. The gel is visualized with ethidium bromide. The data indicate that the removal of the LTR intron activated the ψ-SL1 region for dimerization in each of the constructs except Tat. The resistance of Tat LTR-spliced RNA dimers to inhibition by asSL1 and asψ oligonucleotides (lanes 16 and 17) was investigated further by using the additional oligonucleotide asTAT targeted against an autocomplementary sequence near the 3′ end of this construct. Lanes 18 and 19 indicate that this autocomplementary sequence was responsible for this dimerization. (B) Plot of the percentage of tight dimerization at 55°C as a function of the presence of oligonucleotides. The y-axis error bars represent the standard deviation of two experiments shown in (A).