Table 1. Variability of hybridization.
| Heat-shocked RNA | Control RNA | |||
|---|---|---|---|---|
| Numbera | CV% | Numbera | CV% | |
| Duplicate spots within an array | ||||
| Average | 32 | 5.7 | 32 | 6.6 |
| Normalized signal < 1.0 | 19 | 5.9 | 20 | 7.2 |
| Normalized signal > 1.0 | 13 | 5.4 | 12 | 5.8 |
| Four arrays within a chip | ||||
| Average | 32 | 8.7 | 32 | 9.0 |
| Normalized signal < 1.0 | 19 | 9.7 | 20 | 10.5 |
| Normalized signal > 1.0 | 13 | 7.9 | 12 | 7.4 |
| Twelve arrays among three chips | ||||
| Average | 32 | 10.3 | 32 | 12.8 |
| Normalized signal < 1.0 | 19 | 11.8 | 20 | 15.5 |
| Normalized signal > 1.0 | 13 | 9.1 | 12 | 9.3 |
aThe number of genes scored as positive signals on the array.
CV, coefficient of variation.