. 2016 Aug;114:T5.

TABLE 1.

CHARACTERISTICS OF PRIMARY TUMORS AND CELL LINES^*

	MATERIAL

CHARACTERISTIC	PRIMARY TUMOR 92-1 (IIA)	CELL LINE 92-1	PRIMARY TUMOR E-143-90 (MEL202)	CELL LINE MEL202	PRIMARY TUMORE-1268-94 (MEL270)	CELL LINE MEL270	PRIMARY TUMOR E-994-95 (MEL285)	CELL LINE MEL285	PRIMARY TUMOR E-131-96 (MEL290)	CELL LINE MEL290
	Exenteration		I-125		I-125				nevus of Ota
			choroidal, very pigmented		choroidal, large, into optic nerve; nevoid area		ciliochoroidal, mushroom		choroidal, dome, many vessels
GNA11ex4, ex5	WT^L,R	WT²⁶,²⁸,^R		WT²⁸	WT^L,R	WT²⁸,^R	Q209 L^R,L	WT²⁶,^R	WT^L	WT²⁶,^R
GNAQ ex4, ex5	Q209L 626 A>T	Q209L 626 A>T²⁶,^R		Q209L 626 A>T R210K²⁶ 629 G>A²⁸	Q209P^L,R	Q209P 626 A>C²⁸,^R	WT^L,R	WT²⁶,^R	WT^L	WT²⁶,^R
BAP1 mutation		WT^R				WT^R		WT^R		WT^R
BAP1 protein		yes²⁸		yes²⁸		Yes (OMM2.5 yes)²⁸		yes, low^†		yes, low^†
BAP1 IHC	positive	positive^R			negative	positive ^R	negative	positive^R	positive	negative ^R
Chromosome 3	disomy 3¹	disomy 3¹,⁴²		disomy 3²⁷,⁴⁶,^L 51 chrom	monosomy 3 (50%)^R,L	disomy 3^L loss 3p24, loss 3q21.2–3q24⁴⁵	monosomy 3^L	disomy 3²⁷ loss 3p26-pter⁴²	monosomy 3 (80%)^R,L	disomy 3^R,L loss 3p26-pter⁴²,⁴⁴
Chromosome 6	tetrasomy 6p^L	gain 6p⁴²,^L		gain 6p loss 6q	gain 6p^L	tetrasomy 6p⁴²	disomy 6p^L	6q loss⁴²	disomy 3p^L	disomy 6⁴²
Chromosome 8	tetrasomy 8^R,L	gain 8q⁴²,^L		gain 8q (6 copies)^L	disomy 8^R,L	disomy 8q^L extra 8⁴²	disomy 8q^L	disomy 8p^R tetrasomy 8q^R,L	disomy 8^R,L	disomy 8^R,L /8q24.1-24.2 gain⁴²
EIF1AX		c.17G/A²⁸,^R		WT²⁸		WT^R		WT^R		WT^R
SF3B1 exon 12–16		WT²⁸,^R		C1793c>T²⁸		WT^R		WT^R		WT^R
Cell type			90% epithelioid		mixed, many vessels		mixed, 20% epithelioid		95% epithelioid, many vessels
HMB45 (gp100)	positive	positive⁴⁰,⁴³		positive⁴⁰,⁴³	99% 3+ nevus 50%	positive⁴¹	99% 3+	absent⁴³	99% 3+	absent⁴³
Melan-A		positive²⁶,⁴³			80% 1+ nevus 0%	positive²⁶,²⁸	80% 2–3+	absent⁴³	95% 3+	absent⁴³
CD68	3+				3+				3+
CD3	1–2+				1+		1+		2+
Pigment macrophages			many		many		sporadic		many

Data regarding the primary choroidal melanoma and the choroidal melanoma cell lines were collected from published papers. A cell is empty when information is not available. The presence of mutations in exons 4 and 5 of GNAQ and GNA11 was studied in the primary choroidal melanomas and in the choroidal melanoma cell lines (R = Rotterdam, L = Leiden). Mutations in E-1268-94 and E-994-95 were also determined in Liverpool with the same results. Mutations in exons 1 and 2 of EIF1AX and exons 12–16 of SF3B1 were determined in 92-1, Mel270, Mel285, and Mel290 (Rotterdam). The chromosome composition of the primary tumors and the choroidal melanoma cell lines was obtained from the literature or obtained by FISH analysis or Droplet PCR (primary tumors) or SNP analysis (cell lines). Immunohistochemical analyses were performed for expression of BAP1, HMB45, Melan-A, and the presence of macrophages (CD8) and T cells (CD3) on sections of the primary uveal melanoma.

^†

As determined by Dr. A. G. Jochemsen, LUMC, Leiden, The Netherlands.