TABLE 3.
HLA TYPE OF PATIENTS AND CELL LINES*
| HLA POLYMORPHISMS | ||||||
|---|---|---|---|---|---|---|
| CELL LINE | HLA-A | HLA-B | HLA-C | HLA-DR | HLA-DRw | HLA-DQ |
| 92-1 (PBLs) | 2, 3 | 44 (12), 51 (5), 7B, Bw4 | 501, 14 | 4 | 53 | 3,7, 8 |
| 92-1 cell line (DNA) | 0201, 0301 | 4402, 5101 | 0501, 1402 | 0401 | 4 | 03 |
| Expressed | 2, 3 | B5 (not B44) | ||||
| Mel202 (PBLs) | 1, 3 | 16, 38, 52, Bw4 | -,- | 13, 15 | 51, 53 | 5, 6 |
| Mel202 cell line (DNA) | 0101, 0301 | 3801, 5201 | 1202, 1203 | 1301, 1501 | 3, 5 | 0601, 0603 |
| Expressed | 1, 3 | B38 (not B5) | ||||
| Mel270 (primary tumor E-1268-94) | 11, 29 | 7, 52, Bw4, Bw6 | 13, 15 | 51, 52 | 6 | |
| Mel270 cell line (DNA) | 1101, 2901 | 0705, 5201 | 1202, 1505 | 1301, 1501 | 3, 5 | 0601, 0603 |
| Expressed | 11, 29 | 5, 27 | ||||
| Mel285 (primary tumor E-994-95) | 24 | 7, (53), Bw4, Bw6 | Cw1 | 4 | 51, 53 | 3, 6 |
| Mel285 cell line (DNA) | 2402 | 1302 | 0602 | 0701 | 4 | 0201 |
| Expressed | 24 | 5, 27 (not 7) | ||||
| Mel290 (primary tumor E-131-96) | 1, 2 | 57, Bw4 | Cw6, Cw12 | 13 | 52 | (6), (7) |
| Mel290 cell line (DNA) | 0101, 0201 | 3801, 5701 | 0602, 1203 | 1301, 1305 | 3 | 0301, 0603 |
| Expressed | 1, 2 | 5 (not 7) | ||||
*
HLA types as determined in Leiden on peripheral blood leukocytes (92-1), in Boston on peripheral blood leukocytes (Mel202, HLA Class II of Mel 270), or on material acquired from the primary choroidal melanoma (according to the nomenclature of 1997, Verbik35), and on the cell lines (in Leiden). Empty cells indicate that a certain HLA type has not been tested.
-,- indicates an unsuccessful test.
Genotyping of the cell lines in Leiden47 showed for 92-1: A*0206, A*03, B*05, B*44, B*04; for Mel202 A*01, A*03, B*05, B*38, B*04; for Mel270 A*11, A*29, B*7, B*52 (5), B*04, B*06; for Mel285 A824, B*7, B*53, B*04, B*06; for Mel290 A81, A*2, B*57 (17), B*04.