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Changes in peripheral blood mononuclear cells (PBMCs) by flow cytometry. An increased frequency of activated T cells (as indicated by high ICOS expression) and proliferating T cells (as indicated by Ki67-positivity) is observed in peripheral blood mononuclear cells at 30 days post-mastectomy follow-up versus baseline in cryo plus ipi treated groups. Medians with interquartile ranges are depicted. Abbreviations: cryo, cryoablation; ipi, ipilimumab, ICOS, inducible co-stimulator.
