. Author manuscript; available in PMC: 2017 Dec 6.

Published in final edited form as: Biochemistry. 2016 Nov 28;55(48):6718–6729. doi: 10.1021/acs.biochem.6b01014

Table 1.

PCR primers used to generate HDAC8 mutants

Mutation	Direction	Nucleotide Sequence^a
G302A	forward	5′–CT ACC CTG ATC CTG GCT GGC GGT GGT TAC AAC–3′
G302A	reverse	5′–GTT GTA ACC ACC GCC AGC CAG GAT CAG GGT AG–3′
G303A	forward	5′–CTG ATC CTG GGT GCC GGT GGT TAC AAC C–3′
G303A	reverse	5′–G GTT GTA ACC ACC GGC ACC CAG GAT CAG–3′
G304A	forward	5′–G ATC CTG GGT GGC GCT GGT TAC AAC CT–3′
G304A	reverse	5′–CAG GTT GTA ACC AGC GCC ACC CAG GAT C–3′
G305A	forward	5′–CTG GGT GGC GGT GCT TAC AAC CTG GCG AAC–3′
G305A	reverse	5′–GTT CGC CAG GTT GTA AGC ACC GCC ACC CAG–3′

Boldface letters indicate the introduced mutant codon.