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Sequence of the customized overhangs for cloning into the different cassettes.
| Cassette 1 | Cassette 2 | Cassette 3 | Cassette 4 | |
|---|---|---|---|---|
| Sequence 5′ to 3′ | CACCGG[target]GT | ACCGG[target]G | CCGG[target] | ACACCGG[target] GTT |
| Sequence 5′ to 3′ | TAAAAC[rc-target]CC | AAAAC[rc-target]C | A AAC[rc-target] | CTAAAAC[rc-target]CCG |
