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. 2016 Oct 20;8(6):165–176. doi: 10.1080/19382014.2016.1246637

Figure 2.

Figure 2.

Culture at half standard glucose increases levels of mitochondrial complex proteins. Levels of protein subunits of complexes I and II were measured by Western blotting. (A) shows protein levels after culture at half standard (1/2 Std) vs. standard (Std) glucose (G) (for rat islets and cells 1/2 Std = 5.5 mM G and for human islets 1/2 Std = 2.75 mM G). Data are mean ± SEM of 8 (rat islets), 3 (human islets) and 9 (INS-1 832/13 cells) experiments, **p < 0.002 and *p < 0.03 vs. protein levels after culture at standard glucose. (B) Representative Western blots are shown of levels of complex I and complex II at standard (Std) and half standard (1/2 Std) glucose (G). Band images from human islet experiments were from separate parts of the same gel.