Table 1.
Insulin release
| Insulin release (μU/islet and for cells μU/μg protein) | ||||||
|---|---|---|---|---|---|---|
| Rat islets (n = 4) | Inc. G (mM) | Inc. G (mM) | Inc. G (mM) | |||
| Prev. G (mM) | ||||||
| 11 | 0.4 ± 0.0 | 3.3 | 100.3 ± 6.9 | 16.7 | ||
| 5.5 | 0.7 ± 0.2 | 3.3 | 89.3 ± 10.9 | 16.7 | ||
| Human islets (n = 4) | ||||||
| Prev. G (mM) | ||||||
| 5.5 | 1.4 ± 0.8 | 1.6 | 14.1 ± 4.3 | 16.7 | ||
| 2.75 | 1.9 ± 1.1 | 1.6 | 16.0 ± 6.9 | 16.7 | ||
| INS-1 832/13 (n = 5) | ||||||
| Prev. G (mM) | ||||||
| 11 | 0.13 ± 0.01 | 3.3 | 0.35 ± 0.06 | 11 | 0.37 ± 0.06 | 27 |
| 5.5 | 0.26 ± 0.04* | 3.3 | 0.46 ± 0.11* | 11 | 0.64 ± 0.24* | 27 |
Insulin release after previous (Prev.) culture at standard or half standard glucose (G) as investigated in final incubations (Inc.). Data are mean ± SEM, *p< 0.05 for the effect of previous culture at 5.5 vs. 11 mM G in INS-1 cells.