Table 2.

Assessment of apoptosis and necrosis in rat islets and INS-1 832/13 cells.

	Hypoxia-reoxygenation-induced increase in DNA fragments (for islets fold increased DNA and for cells incremental DNA).
Rat islets	Subjected to 0.8% O2 for 5.5 h (n = 4)		Subjected to 2.8% O2 for 5.5 h (n = 4)
Previous glucose (mM)	Apoptosis	Necrosis	Apoptosis	Necrosis
11	10.00 ± 3.70	15.50 ± 2.00	3.22 ± 0.42	1.65 ± 0.29
5.5	12.20 ± 5.00	12.10 ± 0.70	2.54 ± 0.61	1.31 ± 0.16
INS-1 832/13 cells	Subjected to 0.3-0.5% O2 for 18 h (n = 8)
Previous glucose (mM)	Apoptosis	Necrosis
11	2.42 ± 0.19	2.31 ± 0.26
5.5	1.52 ± 0.14*	1.52 ± 0.27

For rat islets data are fold increased DNA vs. levels at normoxia, mean ± SEM. For INS-1 832/13 data are mean ± SEM of incremental DNA.

^*p < 0.02 for the effect of culture at 5.5 vs. 11 mM glucose.