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Oleate induces PAK4 phosphorylation in human islets. (A) Protein extracts from human islets stimulated for 5 min with 5 mM glucose with or without 0.5 mM oleate were analyzed by Western blot for phospho-PAK4 (S99) and tubulin. (B) Quantification of phospho-PAK4 (S99) normalized to tubulin. Data are mean ± SEM for 4 independent experiments; *, p < 0 .05 compared to 5 mM glucose.
