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FTIR spectra of CO complexes formed with reduced PHM WT. (a) CO complexes of reduced PHM WT in the presence (green) and absence (black) of peptide substrate Ac-YVG. (b) PHM WT-CO complexed with increasing ratios of peptide substrate Ac-YVG; 0.5 equivalents in red; 2 equiv in purple; 4 equiv in black. The IR data were normalized to the protein concentration.
