Figure 3.

Comparison of the raw CO band intensities of PHM WT with increasing amounts of peptide substrate Ac-YVG at 2092 cm⁻¹ and 2063 cm⁻¹. (a) Zero equivalents of peptide substrate added in gray; 0.5 equivalents in red; 2 equiv in purple; 4 equiv in black. (b–c) The IR data in the bar graphs were plotted without normalizing the CO peak intensities. (b) Bar graph comparing the CO intensities at 2092 cm⁻¹ (black) and 2063 cm⁻¹ (red) of reduced PHM WT as increasing amounts of substrate are added. (c) The total peak intensity for both 2092 cm⁻¹ (black) and 2063 cm⁻¹ (red) was plotted as a stacked bar graph for WT PHM-CO with increasing peptide substrate ratios.