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FTIR spectra of the CO complexes of PHM CuH site variant M109I in the presence and absence of substrate Ac-YVG. M109I with substrate (magenta) and without substrate (black). In the absence of peptide substrate, spectra are normalized to the intensity of the CO band at 2092 cm−1. In the presence of substrate, M109I was normalized to 2063 cm−1.
