. Author manuscript; available in PMC: 2017 Dec 6.

Published in final edited form as: Biochemistry. 2016 Nov 22;55(48):6652–6661. doi: 10.1021/acs.biochem.6b00845

Table 1.

Infrared Frequencies and Copper Stoichiometries for Carbonyl Complexes of PHMcc WT and Variants

protein	ν(CO) 1	ν(CO) 2	Cu:Protein
PHM WT	2092		2.02 ± 0.15
PHM WT + Ac-YVG	2092	2063
PHM WT + Hippuric Acid	2093	2075
H172A²⁴	2092		1.4 ± 0.3
H172A + Ac-YVG	2092	2065
M314H	2075		1.6 ± 0.28
M314H + Ac-YVG	2075	2052
M109I	2092		2.08 ± 0.14
M109I + Ac-YVG	2092	2063
H107A	2092		2.01 ± 0.01
H107A + Ac-YVG	2092
H108A	2093		2.04 ± 0.23
H108A + Ac-YVG	2087
H107AH108A	2090		1.09 ± 0.15
H107AH108A + Ac-YVG	2083
H242A²⁴	no peak		1.11 ± 0.03
M314I¹⁹	no peak		1.2 ± 0.38