(A) Immunoblot for co-immunoprecipitated HA-GIPR-GFP blotted for HA-GIPR-GFP and βA2. IP was done with or without GIP stimulation. (Right) Quantification of co immunoprecipitation experiments like that shown in the left panel. βA2 in each lane was normalized to its input and to the GIPR in the IP. Data are averages ± SD of 3 independent experiments., P<0.05.
(B) Immunoblot for co-IP of HA-GIPR-GFP in GRK 2+5 KD cells. Blot was done for HA-GIPR-GFP and βA2. (Right) Quantification of co immunoprecipitation experiments like that shown in the left panel. βA2 in each lane was normalized to its input and to the GIPR in the IP.