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. 2016 Dec 22;1(21):e86654. doi: 10.1172/jci.insight.86654

Figure 1. Autophagic activity is increased in preterm placentas.

Figure 1

(A) Representative images of immunohistochemical staining of LC3 and P62 in placentas of early preterm (n = 10), late preterm (n = 10), and term deliveries (n = 20). Scale bar: 200μm. (B) Quantification of LC3 and P62 immunohistochemical staining. Staining images were examined and scored in a blinded fashion. Intensity of staining was scored from 1 (low) to 5 (high, P62) or 6 (high, LC3). (C) Western blot analysis of LC3-II, P62, ATG7, ATG16L1, BECLIN-1, and ACTIN from human placental samples from the indicated groups. (D–H) Quantification of indicated autophagy proteins normalized to ACTIN. (I) White blood cell (WBC) counts of patients in indicated groups. Data are expressed as mean ± SEM in B–I. *P < 0.05, **P < 0.01 using Kruskal-Wallis test with Dunnett’s post-test.